{"id":103,"date":"2019-07-10T11:37:16","date_gmt":"2019-07-10T14:37:16","guid":{"rendered":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/?page_id=103"},"modified":"2019-07-10T11:37:18","modified_gmt":"2019-07-10T14:37:18","slug":"nanodrop","status":"publish","type":"page","link":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/nanodrop","title":{"rendered":"Nanodrop"},"content":{"rendered":"\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 12pt\"><span style=\"color: #000000;font-size: 10pt\"><span style=\"font-weight: bold\">O NanoDrop ND-1000 \u00e9 um espectrofot\u00f4metro de espectro completo (220-750nm) que quantifica amostras de DNA, RNA e prote\u00ednas com alta reprodutibilidade e precis\u00e3o.\u00a0<\/span><\/span><\/span><\/p>\n<hr style=\"height: 0px;margin-top: 20px;margin-bottom: 20px;border-bottom: 0px;border-top-color: #eeeeee;color: #333333;font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;font-size: 14px\" \/>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\">\u00a0<span><span style=\"font-weight: bold;color: #004074\">NORMAS DE USO<\/span><\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\"><span style=\"font-weight: bold\"><span style=\"color: #003366\">Antes de utilizar este equipamento, consultar as &#8220;normas gerais de uso&#8221;<\/span><\/span><\/span><\/p>\n<p style=\"margin-top: 0px;margin-bottom: 10px;color: #333333;font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;font-size: 14px\"><span style=\"font-size: 10pt;background-color: #ffff00\"><span style=\"font-weight: bold\"><span style=\"font-size: 12pt\">Aten\u00e7\u00e3o:<\/span>\u00a0\u00c9 obrigat\u00f3rio o uso de JALECO e LUVAS\u00a0<span>SEM TALCO<\/span>\u00a0para utiliza\u00e7\u00e3o do NanoDrop.<\/span><\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">1. Os usu\u00e1rios s\u00e3o respons\u00e1veis pelo experimento, principalmente no que se refere ao m\u00e9todo e \u00e0 verifica\u00e7\u00e3o da operacionalidade do equipamento.<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"color: black;font-family: Arial, sans-serif;font-size: 10pt;line-height: 21.6pt\">2. Os materiais e os reagentes que ser\u00e3o necess\u00e1rios para a realiza\u00e7\u00e3o do experimento s\u00e3o de responsabilidade, \u00fanica e exclusiva, do usu\u00e1rio.\u00a0<span><span style=\"font-weight: bold\">\u00c9\u00a0<span style=\"font-size: 13.3333px;line-height: 28.8px\">imprescind\u00edvel<\/span>\u00a0que os usu\u00e1rios tragam seus pr\u00f3prios pipetadores, ponteiras e\u00a0<span style=\"font-family: helvetica;font-size: 13.3333px;line-height: 20px\">len\u00e7o macio (<\/span><span style=\"font-family: helvetica;font-size: 13.3333px;line-height: 19.5px\"><i style=\"line-height: 18.24px;font-size: 12.16px\">kimwipes<\/i><\/span><\/span><\/span><span style=\"color: #666666;font-family: helvetica;font-size: 13.3333px;line-height: 20px\"><span style=\"color: #000000\"><span style=\"font-weight: bold\">).<\/span><\/span><br \/><\/span><\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">3. Por quest\u00e3o de espa\u00e7o f\u00edsico, somente ser\u00e1 permitida a perman\u00eancia de dois usu\u00e1rios no equipamento.<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">4. Atrasos para o in\u00edcio da leitura, quando implicarem em preju\u00edzos no hor\u00e1rio subsequente,\u00a0<span style=\"font-weight: bold\">N\u00c3O ser\u00e3o tolerados<\/span>.\u00a0<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">5. Em caso de desrespeito injustificado aos hor\u00e1rios das reservas (falta ou atraso) e\/ou o n\u00e3o cumprimento das normas de uso do equipamento, o usu\u00e1rio ser\u00e1 advertido Corpo T\u00e9cnico do BioRep, podendo ter restrito ou suspenso o seu acesso ao laborat\u00f3rio.<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">6. Qualquer erro ou problema que venha ocorrer com o equipamento, dever\u00e1 ser obrigatoriamente informado ao Corpo T\u00e9cnico do BioRep.<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">7.<span><span style=\"font-weight: bold\"><span>\u00a0Ao iniciar o programa do nanodrop respeitar as instru\u00e7\u00f5es de rotina que aparecem automaticamente (carregar o pedestal com \u00e1gua antes de clicar &#8220;ok&#8221; na mensagem inicial; Essa etapa n\u00e3o exclui a necessidade de calibrar com o &#8220;blank&#8221; da rea\u00e7\u00e3o).<\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">8. Para evitar danos ao equipamento, \u00e9 necess\u00e1rio posicionar apropriadamente o pedestal superior antes de iniciar a leitura (<em>measure<\/em>).\u00a0<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">9. Efetuar a limpeza dos pedestais com len\u00e7o\u00a0<em>kimwipes<\/em>\u00a0entre as leituras.<\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\">10. Ao finalizar as leituras, manter um\u00a0<span style=\"font-size: 13.3333px;line-height: 28.8px\"><span style=\"font-weight: bold\"><span style=\"font-family: helvetica;font-size: 13.3333px;line-height: 20px\">len\u00e7o macio (<\/span><span style=\"font-family: helvetica;font-size: 13.3333px;line-height: 19.5px\"><i style=\"line-height: 18.24px;font-size: 12.16px\">kimwipes<\/i><\/span><\/span><\/span><span style=\"color: #666666;font-family: helvetica;font-size: 13.3333px;line-height: 20px\"><span style=\"color: #000000\"><span style=\"font-weight: bold\">) protegendo os pedestais de detec\u00e7\u00e3o.<\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin: 0cm 0cm 0.0001pt;color: #666666;font-family: Helvetica, Arial, sans-serif;font-size: 13px;line-height: 21.6pt;vertical-align: top;text-align: justify\"><span style=\"font-size: 10pt;font-family: Arial, sans-serif;color: black\"><span style=\"color: #666666;font-family: helvetica;font-size: 13.3333px;line-height: 20px\"><span style=\"color: #000000\">11.<span style=\"font-weight: bold\">\u00a0Desligar o computador e preencher a ficha de uso do equipamento.<\/span><\/span><\/span><\/span><\/p>\n<p><a href=\"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-content\/uploads\/sites\/672\/2019\/07\/Nanodrop_ND-1000_v3.7_Users_manual.pdf\">Clique aqui para acessar o manual de uso do equipamento<\/a><\/p>\n<p><a href=\"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-content\/uploads\/sites\/672\/2019\/07\/POP_003_-_Nanodrop.pdf\">Clique aqui para acessar o Procedimento Operacional Padr\u00e3o do equipamento<\/a><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-106 size-medium\" src=\"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-content\/uploads\/sites\/672\/2019\/07\/P6193508-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.ufsm.br\/app\/uploads\/sites\/672\/2019\/07\/P6193508-300x225.jpg 300w, https:\/\/www.ufsm.br\/app\/uploads\/sites\/672\/2019\/07\/P6193508-768x576.jpg 768w, https:\/\/www.ufsm.br\/app\/uploads\/sites\/672\/2019\/07\/P6193508-1024x768.jpg 1024w, https:\/\/www.ufsm.br\/app\/uploads\/sites\/672\/2019\/07\/P6193508.jpg 1920w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>O NanoDrop ND-1000 \u00e9 um espectrofot\u00f4metro de espectro completo (220-750nm) que quantifica amostras de DNA, RNA e prote\u00ednas com alta reprodutibilidade e precis\u00e3o.\u00a0 \u00a0NORMAS DE USO Antes de utilizar este equipamento, consultar as &#8220;normas gerais de uso&#8221; Aten\u00e7\u00e3o:\u00a0\u00c9 obrigat\u00f3rio o uso de JALECO e LUVAS\u00a0SEM TALCO\u00a0para utiliza\u00e7\u00e3o do NanoDrop. 1. Os usu\u00e1rios s\u00e3o respons\u00e1veis pelo [&hellip;]<\/p>\n","protected":false},"author":115,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-103","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/pages\/103","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/users\/115"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/comments?post=103"}],"version-history":[{"count":0,"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/pages\/103\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/media?parent=103"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/categories?post=103"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ufsm.br\/laboratorios\/biorep\/wp-json\/wp\/v2\/tags?post=103"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}